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Alteration in TRPV 1 and M uscarinic ( M 3) receptor expression and function in idiopathic overactive bladder urothelial cells
Author(s) -
Birder L. A.,
WolfJohnston A. S.,
Sun Y.,
Chai T. C.
Publication year - 2013
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/j.1748-1716.2012.02462.x
Subject(s) - trpv , muscarinic acetylcholine receptor , overactive bladder , chemistry , urothelial cell , capsaicin , muscarinic acetylcholine receptor m3 , endocrinology , trpv1 , urothelium , medicine , receptor , acetylcholine , atropine , transient receptor potential channel , urinary bladder , pathology , alternative medicine
Aim To examine function of both cholinergic (muscarinic) and TRPV 1 receptors in human bladder urothelial ( HBUC ) from non‐neurogenic overactive bladder ( OAB ) patients as compared to control subjects. Methods Primary HBUC cultures were derived from cystoscopic biopsies from OAB and control subjects. Muscarinic and TRPV 1 function was assessed by acetylcholine (5 μ m ) or capsaicin (0.5 μ m ) evoked ATP release, measured by luciferase assay. Overall, expression of TRPV 1 and muscarinic M 3 receptors in bladder urothelial cells was accomplished using western immunoblotting. Results Our findings revealed that the response to acetylcholine in OAB HBUC cultures (which was blocked by the nonselective muscarinic antagonist, atropine methyl nitrate or AMN ) was not significantly different than from controls. The acetylcholine M 3 receptor was slightly decreased as compared to control. In contrast, OAB HBUC cultures exhibited a capsaicin hypersensitivity and augmented release of ATP (3.2 fold higher), which was blocked by the antagonist capsazepine. The increase in capsaicin sensitivity correlated with increased urothelial TRPV 1 expression. Conclusion Though characterized in a small number of subjects, augmented release of urothelial‐derived transmitters such as ATP could ‘amplify’ signalling between and within urothelial cells and nearby afferent nerves.