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Effect of α‐thalassaemia on exercise‐induced oxidative stress in sickle cell trait
Author(s) -
Faёs C.,
Martin C.,
Chirico E. N.,
Féasson L.,
OyonnoEnguelle S.,
Dubouchaud H.,
Francina A.,
Thiriet P.,
Pialoux V.,
Messonnier L.
Publication year - 2012
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/j.1748-1716.2012.02434.x
Subject(s) - malondialdehyde , oxidative stress , medicine , sickle cell trait , nitrotyrosine , endocrinology , nitric oxide , nitric oxide synthase , disease
Aim Alpha‐thalassaemia is known to reduce intra‐erythrocyte H b S (sickle haemoglobin) concentration in sickle cell trait ( SCT ) subjects. Because H b S was shown to increase oxidative stress, the purpose of this study was to assess the effects of the coexistence of α‐thalassaemia and SCT on oxidative stress markers and nitric oxide ( NO ) metabolism after an acute physical exercise. Methods Forty subjects (age: 23.5 ± 2.21 years), SCT carriers ( H b AS ) or healthy subjects ( H b AA ), with (‐α T ) or without (‐ N α T ) an associated α‐thalassaemia took part in the study. Plasma markers of oxidative stress [advanced oxidation protein products ( AOPP ), protein carbonyl, malondialdehyde ( MDA ) and nitrotyrosine], anti‐oxidant defences and NO metabolism ( NO x) were measured at rest ( T rest ), immediately following an incremental maximal exercise test ( T ex ) and during recovery ( T 1h , T 2h and T 24h ). Results Malondialdehyde expressed as the percentage of changes from baseline was significantly higher in the H b AS ‐ N α T compared with H b AS ‐α T during recovery (+36.3 ± 14.1% vs. −1.8 ± 13.2% at T 1h , P = 0.02; +36.6 ± 13.4% vs. −11.4 ± 12.5% at T 2h , P = 0.004 and +24.1 ± 12.3% vs. −14.4 ± 11.5% at T 24h , P = 0.02 in H b AS ‐ N α T vs. H b AS ‐α T ). Compared with H b AS ‐ N α T , H b AS ‐α T had a higher NO x change from baseline at T ex (−23.4 ± 20.6% vs. +57.7 ± 19.3%, respectively; P = 0.005) and lower nitrotyrosine change from baseline at T 1h (+7.2 ± 22.2% vs. +93.5%±29.3%, respectively; P = 0.04). Conclusion All these data suggest that the presence of α‐thalassaemia may blunt the higher level of oxidative stress and the impaired bioavailability of NO observed in the SCT carriers.