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Leptin regulates sugar and amino acids transport in the human intestinal cell line C aco‐2
Author(s) -
Fanjul C.,
Barrenetxe J.,
Iñigo C.,
Sakar Y.,
Ducroc R.,
Barber A.,
Lostao M. P.
Publication year - 2012
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/j.1748-1716.2012.02412.x
Subject(s) - leptin , brush border , amino acid , transporter , chemistry , biochemistry , caco 2 , apical membrane , membrane transport , biology , cell , endocrinology , vesicle , membrane , gene , obesity
A im Studies in rodents have shown that leptin controls sugars and glutamine entry in the enterocytes by regulating membrane transporters. Here, we have examined the effect of leptin on sugar and amino acids absorption in the human model of intestinal cells C aco‐2 and investigated the transporters involved.M ethods Substrate uptake experiments were performed in C aco‐2 cells, grown on plates, in the presence and the absence of leptin, and the expression of the different transporters in brush border membrane vesicles was analysed by W estern blot. Results Leptin inhibited 0.1 m m α‐methyl‐ D ‐glucoside uptake after 5 or 30 min treatment and decreased SGLT 1 protein abundance in the apical membrane. Uptake of 20 μ m glutamine and 0.1 m m phenylalanine was also inhibited by leptin, indicating sensitivity to the hormone of the N a + ‐dependent neutral amino acid transporters ASCT 2 and B 0 AT 1. This inhibition was accompanied by a reduction in the transporters expression at the brush border membrane. Leptin also inhibited 1 m m proline and β‐alanine uptake in N a + medium at p H 6, conditions for optimal activity of the H + ‐dependent neutral amino acid transporter PAT 1. In this case, abundance of PAT 1 in the brush border membrane after leptin treatment was not modified. Interestingly, leptin inhibitory effect on β‐alanine uptake was reversed by the PKA inhibitor H ‐89 suggesting involvement of PKA pathway in leptin's regulation of PAT 1 activity. Conclusion These data show in human intestinal cells that leptin can rapidly control the activity of physiologically relevant transporters for rich‐energy molecules, that is, D ‐glucose ( SGLT 1) and amino acids ( ASCT 2, B 0 AT 1 and PAT 1).