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Angiotensin II regulates endothelial cell migration through calcium influx via T‐type calcium channel in human umbilical vein endothelial cells
Author(s) -
Martini A.,
Bruno R.,
Mazzulla S.,
Nocita A.,
Martino G.
Publication year - 2010
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/j.1748-1716.2009.02070.x
Subject(s) - mibefradil , t type calcium channel , angiotensin ii , calcium , endocrinology , umbilical vein , calcium channel , medicine , voltage dependent calcium channel , chemistry , calcium in biology , endothelial stem cell , ionomycin , verapamil , angiotensin ii receptor type 1 , trpc1 , receptor , biology , biochemistry , ion channel , in vitro
Aim: The T‐type calcium channel is expressed in vascular endothelial cells, but its role in endothelial cell function is yet to be elucidated. We analysed the endothelial functional role of T‐type calcium channel‐dependent calcium under angiotensin II (Ang II) stimulation. Methods: Human umbilical vein endothelial cells were co‐incubated with hormone at 10 −7 m and either Efonidipine 10 −5 m or Verapamil 10 −5 m or Mibefradil 10 −5 m or Wortmannin 10 −6 m . The contribution of Ang II receptors was evaluated using PD123319 10 −7 m and ZD 7155 10 −7 m . The calcium ion concentration was observed using Fluo‐3 acetossimetil ester. The cells were observed after 3, 6, 9 and 12 h. Results: The microfluorescence method points out that Ang II induces intracellular calcium modulation in time by distinct mechanisms. AT2 receptor blockade is necessary to observe significant increase in [Ca 2+ ] i levels. Pre‐treatment with Mibefradil abolishes Ang II ‐induced cell migration. Conclusions: Our data show that Ang II, via AT1 receptor, modulates calcium concentration involving T‐type calcium channel and L‐type calcium channel but only the calcium influx via T‐type calcium channels regulates endothelial cell migration which is essential for angiogenesis.