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Measuring secretion in chromaffin cells using electrophysiological and electrochemical methods
Author(s) -
Borges R.,
Camacho M.,
Gillis K. D.
Publication year - 2008
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/j.1748-1716.2007.01814.x
Subject(s) - amperometry , electrophysiology , exocytosis , chromaffin cell , neuroscience , chemistry , secretion , biology , electrochemistry , catecholamine , electrode , biochemistry , adrenal medulla
Our present understanding of exocytosis of catecholamines has benefited tremendously from the arrival of single‐cell electrochemical methods (amperometry and voltammetry), electrophysiological techniques (whole‐cell and patch capacitance) and from the combination of both techniques (patch amperometry). In this brief review, we will outline the strengths and limitations of amperometric and electrophysiological methods and highlight the major contribution obtained with the use of these techniques in chromaffin cells.