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Heat shock factor activation in human muscles following a demanding intermittent exercise protocol is attenuated with hyperthermia
Author(s) -
Palomero J.,
Broome C. S.,
Rasmussen P.,
Mohr M.,
Nielsen B.,
Nybo L.,
McArdle A.,
Drust B.
Publication year - 2008
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/j.1748-1716.2007.01774.x
Subject(s) - hyperthermia , shock (circulatory) , medicine , hsp70 , physical exercise , endocrinology , heat shock , heat shock protein , chemistry , biochemistry , gene
Aim: The present study investigated whether increased activation of heat shock factors (HSF) following exercise relates primarily to the increased muscle temperature or to exercise in general. Methods: Six subjects completed 40 min of intermittent cycling (15s : 15s exercise:recovery at 300 ± 22 W) at an ambient temperature of either 20.0 ± 1.3 or 40.3 ± 0.7 °C. Muscle biopsies were taken prior to and immediately following the exercise protocol with samples analysed for HSF DNA binding by electrophoretic mobility shift assay. Results: Exercise at 40 °C resulted in significantly increased oesophageal (39.3 ± 0.2 °C) and muscle temperature (40.0 ± 0.2 °C) at the end of the exercise protocol compared with 20 °C (oesophageal, 38.1 ± 0.1 °C; muscle, 38.9 ± 0.2 °C). However, an increased DNA binding of HSF was not evident following exercise at 40 °C (reduced by 21 ± 22%) whereas it increased by 29 ± 51% following exercise at 20 °C. Conclusion: It appears that increased temperature is not the major factor responsible for activation of HSF DNA binding.