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Treadmill but not wheel running improves fatigue resistance of isolated extensor digitorum longus muscle in mice
Author(s) -
Jeneson J. A. L.,
De Snoo M. W.,
Verlinden N. A. T.,
Joosten B. J. L. J.,
Doornenbal A.,
Schot A.,
Everts M. E.
Publication year - 2007
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/j.1748-1716.2007.01680.x
Subject(s) - treadmill , hindlimb , extensor digitorum longus muscle , stimulation , contraction (grammar) , medicine , chemistry , zoology , anatomy , skeletal muscle , endocrinology , biology
Aim: The present study is the first to compare the physiological impact of either forced treadmill or voluntary wheel running exercise on hindlimb muscle in mice. Methods: Male C57BL/6 mice were subjected to either 6 weeks of forced treadmill or voluntary wheel running exercise. Mice in the treadmill running exercise group (TRE; n = 8) ran 1.9 km day −1 at a speed of 16 m min −1 against an uphill incline of 11 °. In the running wheel exercise group (RWE; n = 8) animals ran 8.8 ± 0.2 km per day (average speed 42 ± 2 m min −1 ). After the experimental period, animals were killed and mechanical performance and oxygen consumption of isolated extensor digitorum longus (EDL) muscle were determined during serial electrical stimulation at 0.5, 1 and 2 Hz. Results: Steady‐state half‐width time (HWT) of twitch contraction at 0.5 Hz was significantly shorter in TRE and RWE than controls (CON) (41.3 ± 0.2, 41.3 ± 0.1 and 44.3 ± 0.1 s respectively; P < 0.05). The rate of fatigue development and HWT lengthening at 2 Hz was the same in RWE and CON but lower in TRE (1.2‐fold and twofold respectively; P < 0.05). EDL oxygen consumption, mitochondrial content and myosin heavy chain (MyHC) composition were not different between the groups. Conclusion: These results indicate that both exercise modalities have an effect on a hindlimb fast‐twitch muscle in mice, with the greatest impact seen with forced treadmill running.