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Muscle type‐specific response of PGC‐1 α and oxidative enzymes during voluntary wheel running in mouse skeletal muscle
Author(s) -
Ikeda S.,
Kawamoto H.,
Kasaoka K.,
Hitomi Y.,
Kizaki T.,
Sankai Y.,
Ohno H.,
Haga S.,
Takemasa T.
Publication year - 2006
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/j.1748-1716.2006.01623.x
Subject(s) - glut4 , skeletal muscle , medicine , endocrinology , oxidative phosphorylation , biology , mitochondrion , soleus muscle , glycolysis , gene expression , tibialis anterior muscle , metabolism , glucose uptake , biochemistry , gene , insulin
Aim: It is generally accepted that endurance exercise increases the expression of peroxisome proliferator‐activated receptor γ coactivator‐1 α (PGC‐1 α ), which governs the expression of oxidative metabolic enzymes. A previous report demonstrated that the regulation of mitochondrial protein expression in skeletal muscles in response to cold exposure depends on muscle fibre type. Cold exposure and endurance exercise are both metabolic challenges that require adjustments in mitochondrial energy metabolism, we hypothesized that the exercise‐induced increase in oxidative enzymes and PGC‐1 α expression is higher in fast‐type than in slow‐type muscle. Methods: Female ICR mice were individually housed in cages equipped with running wheel for 1, 2, 4, 6 or 8 weeks. The soleus, plantaris (PLA) and tibialis anterior (TA) muscles were then prepared from each mouse. The expression levels of PGC‐1 α , mitochondrial proteins and GLUT4 were evaluated by Western blotting. Results: The expression level of PGC‐1 α was increased only in the PLA muscle. Furthermore, the expression levels of all mitochondrial proteins and GLUT4 in the PLA muscle were increased. In the TA muscle, although there was no increase in PGC‐1 α expression, the expression levels of mitochondrial proteins and GLUT4 were increased. Conclusions: These results suggest that muscle type‐specific responses occur during endurance exercise, and that the increase in PGC‐1 α expression is not the only factor that promotes oxidative capacity as a result of endurance exercise.