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Endogenous signalling system involved in parotid gland adenosine A 1 receptor‐amylase release
Author(s) -
Finkelberg A.,
Busch L.,
Reina S.,
SterinBorda L.,
Borda E.
Publication year - 2006
Publication title -
acta physiologica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.591
H-Index - 116
eISSN - 1748-1716
pISSN - 1748-1708
DOI - 10.1111/j.1748-1716.2005.01508.x
Subject(s) - endocrinology , medicine , adenosine , stimulation , receptor , adenosine receptor , biology , protein kinase c , inositol phosphate , agonist , chemistry , inositol , biochemistry , signal transduction
Aim: In this study, we have determined signalling pathways involved in adenosine A 1 receptor (A 1 receptor)‐dependent stimulation of amylase release in rat parotid gland. Methods: Amylase release, binding and cyclic adenosine monophosphate (cAMP) assays, inositol phosphates (IPs) production and nitric oxide synthase (NOS) activity in the presence of cyclopentyl‐1,3‐dipropylxanthine (CPA) alone or in the presence of different inhibitory drugs were performed. Results: The binding parameters of specific A 1 antagonist [ 3 H]‐cyclopentyl 1,3‐dipropilxanthine ([ 3 H]‐DPCPX) in parotid gland membranes show a population of high affinity sites with K d (n m ) 0.53 ± 0.06 and B max (fmol mg −1 protein) 122.6 ± 10.2. CPA stimulation of A 1 receptor exerts an increase in amylase release, IPs accumulation, cAMP production and NOS activity. All these A 1 agonist effects were blocked by the A 1 receptor antagonist DPCPX. Inhibitors of phospholipase C (PLC), calcium/calmodulin (CaM), protein kinase C (PKC), and adenylate cyclase, but not NOS, activities attenuated the CPA stimulatory effect on amylase release. The effect of CPA on amylase release significantly correlated with its action either on cAMP or on IPs accumulation. Conclusion: These results suggest that CPA activation of parotid gland A 1 receptor induces a stimulatory effect on amylase release associated with increased production of cAMP and IPs accumulation. The mechanism appears to occur secondarily to stimulation of phosphoinositide turnover via PLC activation. This, in turn, triggers cascade reactions involving CaM and PKC. The CPA stimulation of NOS does not appear to participate in amylase release.