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Kinetics of Th 17‐related cytokine expression in experimentally induced rat periapical lesions
Author(s) -
Wei Shusheng,
Kawashima Nobuyuki,
Suzuki Noriyuki,
Xu Jing,
Takahashi Satomi,
Zhou Mengyu,
Koizumi Yu,
Suda Hideaki
Publication year - 2013
Publication title -
australian endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.703
H-Index - 34
eISSN - 1747-4477
pISSN - 1329-1947
DOI - 10.1111/j.1747-4477.2012.00371.x
Subject(s) - cytokine , foxp3 , proinflammatory cytokine , immunology , medicine , pathological , inflammation , molar , pathology , dentistry , immune system
Th 17‐related cytokines are essential factors in various pathological states, including inflammatory bone destruction. This study investigated the contribution of Th 17‐related cytokines to the progress of experimentally induced rat periapical lesions. Periapical pathoses were induced by unsealed exposure of the pulp chamber of the lower first molars. A variety of immunocompetent cells, including CD 68 + macrophages, I a antigen + cells and TCR αβ + T cells, were observed in the lesions. The expression levels of Th 17‐related cytokines, IL ‐17 and IL ‐23 , and of pro‐inflammatory cytokines, IL ‐1β and IL ‐6 , were significantly increased at 14 days (expansion stage) compared with normal periapical tissues. The expression levels of F oxp3 , a regulatory T cell ( T reg)‐related gene, and of IL ‐10 , an anti‐inflammatory cytokine, were higher at 28 days (chronic stage) than at 14 days. These findings suggest that Th 17‐related cytokines may be primary contributors to the initiation of periapical bone destruction, and that lesion expansion may be regulated by anti‐inflammatory mediators.