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Evaluation of the antifungal activity of four solutions used as a final rinse in vitro
Author(s) -
Mohammadi Zahed,
Giardino Luciano,
Palazzi Flavio
Publication year - 2013
Publication title -
australian endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.703
H-Index - 34
eISSN - 1747-4477
pISSN - 1329-1947
DOI - 10.1111/j.1747-4477.2010.00278.x
Subject(s) - sodium hypochlorite , candida albicans , chlorhexidine , dentistry , antifungal , chlorhexidine gluconate , agar , chemistry , colony forming unit , in vitro , microbiology and biotechnology , medicine , biology , biochemistry , bacteria , genetics , organic chemistry
The aim of the study was to compare the antifungal activity of 1.3% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), MTAD and Tetraclean as a final rinse against Candida albicans in a human tooth model in vitro . Ninety extracted human maxillary central and lateral incisor teeth were randomly divided into four groups each with 20 teeth, a positive and a negative control each with five teeth. After preparing the root canals, teeth were inoculated with Candida albicans (ATCC 10261) and incubated for 72 h. Teeth were divided into four experimental groups according to the irrigation solution as follows: NaOCl, CHX, MTAD and Tetraclean. After culturing aliquots from the experimental teeth on Sabouraud 4% dextrose agar, colony‐forming units were counted. The results showed that 1.3% NaOCl and 2% CHX were equally effective and significantly superior to MTAD and Tetraclean ( P < 0.05). Furthermore, antifungal efficacy of Tetraclean was significantly superior to MTAD ( P < 0.05).