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Expression of Notch signalling‐related genes in normal and differentiating rat dental pulp cells
Author(s) -
Sun Hantang,
Kawashima Nobuyuki,
Xu Jing,
Takahashi Satomi,
Suda Hideaki
Publication year - 2010
Publication title -
australian endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.703
H-Index - 34
eISSN - 1747-4477
pISSN - 1329-1947
DOI - 10.1111/j.1747-4477.2009.00188.x
Subject(s) - dentin sialophosphoprotein , notch signaling pathway , chemistry , andrology , microbiology and biotechnology , dentin , fetal bovine serum , pulp (tooth) , odontoblast , dentistry , biology , biochemistry , gene , cell , medicine
Notch signalling is of fundamental importance to various processes during embryonic development and in adults. The possible role of Hey1, an important Notch signalling component, in odontoblast differentiation was evaluated in this study. Primary cultured dental pulp cells, derived from upper incisors of 5‐week‐old Wistar rats, were placed in α‐modification of Eagle's minimal essential medium supplemented with 10% Fetal Bovine Serum (FBS), and ascorbic acid (AA) and β‐glycerophosphate (β‐GP), with or without dexamethasone, and cultured on dishes coated with collagen type IA for 7 days. Conventional and real‐time Polymerase Chain Reaction (PCR) was performed to determine the expression of Notch‐related genes and dentin sialophosphoprotein as a marker of odontoblast differentiation. Dentin sialophosphoprotein and Hey1 expression was significantly increased and decreased in the presence of AA + β‐GP compared with controls, respectively. These findings suggest that Hey1 may be a negative regulator in odontoblast differentiation.