Premium
Evaluation of the antibacterial substantivity of several intra‐canal agents
Author(s) -
Khademi Abbas ali,
Mohammadi Zahed,
Havaee Asgar
Publication year - 2006
Publication title -
australian endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.703
H-Index - 34
eISSN - 1747-4477
pISSN - 1329-1947
DOI - 10.1111/j.1747-4477.2006.00033.x
Subject(s) - sodium hypochlorite , chlorhexidine , enterococcus faecalis , doxycycline , antimicrobial , chemistry , root canal , colony forming unit , dentistry , negative control , traditional medicine , medicine , biology , bacteria , antibiotics , escherichia coli , biochemistry , genetics , organic chemistry , gene
The aim of this in vitro study was to compare the antimicrobial substantivity of 2% chlorhexidine gluconate (CHX), 100 mg ml −1 doxycycline and 2.6% sodium hypochlorite (NaOCl) in bovine root dentine. Eighty dentine tubes prepared from bovine incisors were infected in vitro for 14 days with Enterococcus faecalis . The specimens were divided into five groups as follows: doxycycline HCl; CHX; NaOCl; infected dentine tubes (positive control); and sterile dentine tubes (negative control). Dentine chips were collected with round burs into tryptic soy broth. After culturing, the number of colony‐forming units (CFU) was counted. In all experimental groups, the number of CFU was minimum in the first cultures, and the results obtained were significantly different from each other at any time period ( P < 0.05). In the first culture, the NaOCl group and doxycycline HCl group showed the lowest and highest number of CFU, respectively. In each group, the number of CFU increased significantly by time‐lapse ( P < 0.05). In conclusion, the substantivity of CHX was significantly greater than NaOCl and doxycycline.