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Cytotoxicity Of A New Endodontic Filling Material
Author(s) -
Gambarini Gianluca,
AndreasiBassi M.,
Bolognini G.,
Testarelli L.,
Nocca G.,
Ceccarelli L.,
Scatena R.,
Lupi A.,
Castagnola M.
Publication year - 2003
Publication title -
australian endodontic journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.703
H-Index - 34
eISSN - 1747-4477
pISSN - 1329-1947
DOI - 10.1111/j.1747-4477.2003.tb00489.x
Subject(s) - root canal , cytotoxicity , dentistry , neutral red , significant difference , incubation , chemistry , in vitro , medicine , biochemistry
In vitro cell cultures have been widely used as a means of evaluating cytotoxicity of root canal filling materials. Following ANSI/ADA spec. no. 41, the aim of the present study was to investigate the biological compatibility of a new sealer (FibreFill) and compare it with some commercially available endodontic sealers (Bioseal and Acroseal). Mouse 3T3 fibroblasts were seeded and cultured and subsequently extracts of the cements were added. After 24 hours incubation, the cellular vitality of fibroblasts was evaluated by the neutral red uptake test (NRU), which measures the membrane permeability. Data were collected and statistically analysed. Results showed that all tested materials exhibited mild cytotoxic effects, which are compatible with normal clinical use, and no statistically significant difference was noted between FibreFill and the other tested materials. Therefore, selection amongst these sealers should be based on other factors.