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A Combined Study Using Ligand‐Based Design, Synthesis, and Pharmacological Evaluation of Analogues of the Acetaminophen Ortho ‐Regioisomer with Potent Analgesic Activity
Author(s) -
Queiroz Luana M. D.,
Rocha Josmar R.,
Leitão Andrei,
Montanari Carlos A.,
da Silva Albérico B. F.,
Sousa Pergentino J. C.,
Borges Rosivaldo S.
Publication year - 2012
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/j.1747-0285.2012.01372.x
Subject(s) - chemistry , pharmacophore , computational chemistry , analgesic , acetaminophen , stereochemistry , pharmacology , medicine , biochemistry
A ligand‐based drug design study was performed to acetaminophen regioisomers as analgesic candidates employing quantum chemical calculations at the DFT/B3LYP level of theory and the 6‐31G* basis set. To do so, many molecular descriptors were used such as highest occupied molecular orbital, ionization potential, H–O bond dissociation energies, and spin densities, which might be related to quench reactivity of the tyrosyl radical to give N ‐acetyl‐ p ‐benzosemiquinone‐imine through an initial electron withdrawing or hydrogen atom abstraction. Based on this in silico work, the most promising molecule, orthobenzamol, was synthesized and tested. The results expected from the theoretical prediction were confirmed in vivo using mouse models of nociception such as writhing, paw licking, and hot plate tests. All biological results suggested an antinociceptive activity mediated by opioid receptors. Furthermore, at 90 and 120 min, this new compound had an effect that was comparable to morphine, the standard drug for this test. Finally, the pharmacophore model is discussed according to the electronic properties derived from quantum chemistry calculations.