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In Vitro Studies on the Antioxidant and Protective Effect of 2‐Substituted ‐8‐Hydroxyquinoline Derivatives Against H 2 O 2 ‐Induced Oxidative Stress in BMSCs
Author(s) -
Wang TingTing,
Zeng GongChang,
Li XiCan,
Zeng HePing
Publication year - 2010
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/j.1747-0285.2009.00925.x
Subject(s) - chemistry , oxidative stress , antioxidant , dpph , viability assay , hydrogen peroxide , mesenchymal stem cell , lipid peroxidation , mtt assay , biochemistry , in vitro , biology , microbiology and biotechnology
Novel 2‐vinyl‐8‐hydroxyquinoline derivatives as potential antioxidants and regulators of H 2 O 2 ‐induced oxidative stress in rat bone marrow mesenchymal stem cells (MSCs) are first reported. The antiradical properties and the reducing power of these compounds were assessed using 2, 2‐diphenyl‐1‐picrylhydrazyl (DPPH) and auto‐oxidation of pyrogallol method, respectively. The activity against lipid peroxidation was determined using ammonium thiocyanate method. The results revealed that introduction of electron‐donating groups at 2nd position decreased the antioxidant activities of 8‐hydroxyquinoline derivatives. In addition, compound 4 , the structure of which is similar to melatonin, exhibited superior antioxidant activities in scavenging DPPH free radical, ˙O 2 free radical, and anti‐LPO activities. Except for compounds 7 , 12 , and 15 , the other compounds exhibited a stimulatory effect on MSCs growth. Using hydrogen peroxide (H 2 O 2 ), we also investigated the protective efficacy of 2‐vinyl‐8‐hydroxyquinoline derivatives against oxidative stress‐induced cell death of MSCs. Cell viability assayed by MTT method indicated that exposure of MSCs cultures to hydrogen peroxide resulted in a concentration‐dependent decrease in cell viability, and compounds 4 and 5 at given concentration (2.62 × 10 −3 m ) could protect MSCs against H 2 O 2 ‐induced oxidative stress in bone mesenchymal stem cell (BMSCs).