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Immunosuppressive Activity of Buxidin and E ‐Buxenone from Buxus hyrcana
Author(s) -
Mesaik M. Ahmed,
Halim Sobia A.,
UlHaq Zaheer,
Choudhary M. Iqbal,
Shahnaz Salma,
Ayatollahi S. A. M,
Murad Shahnaz,
Ahmad Aqeel
Publication year - 2010
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/j.1747-0285.2009.00906.x
Subject(s) - chemotaxis , docking (animal) , in vitro , cytokine , chemistry , stereochemistry , cell growth , microbiology and biotechnology , biology , biochemistry , pharmacology , receptor , immunology , medicine , nursing
Buxidin ( 1 ) and E ‐Buxenone ( 2 ), steroidal alkaloids from Buxus hyrcana , are found to possess potent immunosuppressive properties. The activity was tested in vitro on oxidative burst, chemotaxis, T‐cell proliferation, and cytokine production. Both compounds showed a significant immunomodulatory activity with clear suppressive effect on oxidative burst and chemotaxis in a dose‐dependent manner. They also exhibited suppressive effect on the phytohemagglutinin‐stimulated T‐cell proliferation. The immunomodulatory activity was further confirmed by the suppression of IL‐2 and IL‐4 production. Furthermore, molecular docking studies were performed to investigate the binding mode of Buxidin ( 1 ) and E ‐Buxenone ( 2 ) with IL‐2. Despite the structural differences between Buxidin ( 1 ) and E ‐Buxenone ( 2 ), docking results revealed that they adopt a similar binding pattern at the active site of the IL‐2. A good agreement between practical and theoretic results indicates that the current docking study could provide an alternate tool for the structural optimization of recently identified ligand as more potent IL‐2 inhibitors.