Premium
Modulatory Effects of 6‐Carboxymethylthiopurine on Activated Murine Macrophages
Author(s) -
De Paula Marcio L.,
Braga Fernanda G.,
Coimbra Elaine S.,
Carmo Arturene M. L.,
Teixeira Henrique C.,
Da Silva Adilson D.,
Souza Maria A.,
Ferreira Ana Paula
Publication year - 2008
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/j.1747-0285.2008.00665.x
Subject(s) - nitric oxide , interferon gamma , phagocytosis , griess test , mycobacterium bovis , microbiology and biotechnology , viability assay , interferon , chemistry , immunology , biology , cell , cytokine , biochemistry , medicine , mycobacterium tuberculosis , pathology , endocrinology , nitrite , tuberculosis , organic chemistry , nitrate
The immunological activity of macrophages against pathogens in hosts includes the phagocytosis and the production of nitric oxide. We report herein the investigation of the effect of 6‐carboxymethylthiopurine on nitric oxide production by murine macrophages as well as its effect on the cell viability and proliferation after stimulus with Mycobacterium bovis bacille Calmette–Guérin, interferon‐gamma or a combination of both. J774A.1 macrophages stimulated or not by bacille Calmette–Guérin (20 μ g/mL), interferon‐gamma or both, were cultured in the presence of 6‐carboxymethylthiopurine (125, 250 and 500 μ m ). Nitric oxide production was measured by the Griess method and cell viability/proliferation by the diphenyltetrazolium assay [3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyltetrazolium bromide]. We observed an increase of J774A.1 cell proliferation after stimulus with bacille Calmette–Guérin at 125, 250 and 500 μ m (69.1, 124.0 and 89.7%, respectively) and with interferon‐gamma at 125 and 250 μ m (64.8% and 61.7%, respectively) (p < 0.05). In all cultures treated with 6‐carboxymethylthiopurine, interferon‐gamma‐activated nitric oxide production by J774A.1 cells decreased as well as when subjected to interferon‐gamma plus bacille Calmette–Guérin stimuli at 500 μ m (p < 0.05). Altogether these data point to an anti‐inflammatory effect of 6‐carboxymethylthiopurine on stimulated macrophages.