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Metabolism of a Highly Selective Gelatinase Inhibitor Generates Active Metabolite
Author(s) -
Lee Mijoon,
VillegasEstrada Adriel,
Celenza Giuseppe,
Boggess Bill,
Toth Marta,
Kreitinger Gloria,
Forbes Christopher,
Fridman Rafael,
Mobashery Shahriar,
Chang Mayland
Publication year - 2007
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/j.1747-0285.2007.00577.x
Subject(s) - metabolite , gelatinases , matrix metalloproteinase , in vivo , chemistry , matrix metalloproteinase inhibitor , biochemistry , hydroxylation , in vitro , pharmacology , gelatinase a , gelatinase , enzyme , biology , microbiology and biotechnology
(4‐Phenoxyphenylsulfonyl)methylthiirane (inhibitor 1 ) is a highly selective inhibitor of gelatinases (matrix metalloproteinases 2 and 9), which is showing considerable promise in animal models for cancer and stroke. Despite demonstrated potent, selective, and effective inhibition of gelatinases both in vitro and in vivo , the compound is rapidly metabolized, implying that the likely activity in vivo is due to a metabolite rather than the compound itself. To this end, metabolism of inhibitor 1 was investigated in in vitro systems. Four metabolites were identified by LC/MS‐MS and the structures of three of them were further validated by comparison with authentic synthetic samples. One metabolite, 4‐(4‐thiiranylmethanesulfonylphenoxy)phenol (compound 21 ), was generated by hydroxylation of the terminal phenyl group of 1 . This compound was investigated in kinetics of inhibition of several matrix metalloproteinases. This metabolite was a more potent slow‐binding inhibitor of gelatinases (matrix metalloproteinase‐2 and matrix metalloproteinase‐9) than the parent compound 1 , but it also served as a slow‐binding inhibitor of matrix metalloproteinase‐14, the upstream activator of matrix metalloproteinase‐2.