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Detection of Sulfur Mustard Adducts in Human Callus by Phage Antibodies
Author(s) -
Bikker Floris J.,
MarsGroenendijk Roos H.,
Noort Daan,
Fidder Alex,
Van Der Schans Govert P.
Publication year - 2007
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/j.1747-0285.2007.00504.x
Subject(s) - sulfur mustard , phage display , antibody , keratin , chemistry , callus , biochemistry , microbiology and biotechnology , biology , immunology , genetics , organic chemistry , toxicity
As part of a research program to develop novel methods for diagnosis of sulfur mustard exposure in the human skin the suitability of phage display was explored. Phage display is a relative new method that enables researchers to quickly evaluate a huge range of potentially useful antibodies, thereby bypassing the more costly and time‐consuming hybridoma technique. The Tomlinson I and J phage libraries were used to select phage antibodies exhibiting affinity for sulfur mustard adducts on keratins, isolated from human callus. Two kinds of phage antibodies were obtained: antibodies recognizing keratin and antibodies recognizing keratin which was exposed to sulfur mustard. These phage antibodies retained activity after repeated culturing and culturing in larger volumes. For the first time antibody phage display was successfully applied for immunodiagnostics of a chemical warfare agent.