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Synthesis of Overlapping Fibrin Citrullinated Peptides and their use for Diagnosing Rheumatoid Arthritis
Author(s) -
Pérez María L.,
Gómara María J.,
Kasi Devi,
Alonso Ana,
Viñas Odette,
Ercilla Guadalupe,
Sanmartí Raimon,
Haro Isabel
Publication year - 2006
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/j.1747-0285.2006.00438.x
Subject(s) - rheumatoid arthritis , autoantibody , fibrin , antigenicity , medicine , epitope , immunology , arthritis , rheumatoid factor , antigen , antibody
With the aim of developing a new enzyme‐linked immunosorbent assay test to detect autoantibodies in the sera of rheumatoid arthritis patients with a high sensitivity and specificity using synthetic citrullinated peptides of fibrin (which is abundant in rheumatoid synovium) as antigenic substract, peptides belonging to α ‐ and β ‐fibrin chains were selected by computer‐aided prediction of antigenicity and epitope mapping and synthesized in solid phase. We analysed by enzyme‐linked immunosorbent assay 133 sera from patients with well‐characterized rheumatic diseases, including 67 patients with rheumatoid arthritis. The results of the immunoassays reported highlight the usefulness of fibrin‐related peptides in rheumatoid arthritis diagnosis and, especially, the ability and specificity of the [Cit 621,627,630 ] α ‐fibrin(617–631) ( α fib617) peptide sequence to recognize the autoantibodies that are present in rheumatoid arthritis patients.