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Evaluation of the Antibacterial Spectrum of Drosocin Analogues
Author(s) -
Bikker Floris J.,
Kamanvan Zanten Wendy E.,
De Vriesvan de Ruit AnneMarij B. C.,
VoskampVisser Ingrid,
Van Hooft Peter A. V.,
MarsGroenendijk Roos H.,
De Visser Peter C.,
Noort Daan
Publication year - 2006
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/j.1747-0285.2006.00424.x
Subject(s) - antibacterial activity , spectrum (functional analysis) , chemistry , computational biology , combinatorial chemistry , biology , bacteria , physics , genetics , quantum mechanics
Drosocin is a 19‐mer, cationic antimicrobial peptide from Drosophila melanogaster . The aim of the study was to examine the antibacterial spectrum of unglycosylated drosocin analogues. Furthermore, the amino acid sequence of DnaK, drosocin's intracellular target, from susceptible species was aligned and studied for sequence homology. From this a panel of 31 bacterial strains, including Salmonella strains with truncated lipopolysaccharide structures, was tested for susceptibility towards three drosocin analogues. Available bacterial DnaK amino acid sequences were retrieved from the ExPASy proteomics server of the Swiss Institute of Bioinformatics studied for sequence homology. Seventeen of the 31 strains tested were susceptible for the drosocin analogues. Minimal inhibitory concentration values against mainly Gram‐negative bacteria ranged from 3.1 to 100 μ m . With the exception of Micrococcus luteus and Xanthomonas campestris all drosocin analogue‐susceptible strains were Enterobacteriaceae showing a high DnaK amino acid sequence homology.