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DNA‐binding by Functionalized Gold Nanoparticles: Mechanism and Structural Requirements
Author(s) -
Goodman Catherine M.,
Chari Nandini S.,
Han Gang,
Hong Rui,
Ghosh Partha,
Rotello Vincent M.
Publication year - 2006
Publication title -
chemical biology and drug design
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.59
H-Index - 77
eISSN - 1747-0285
pISSN - 1747-0277
DOI - 10.1111/j.1747-0285.2006.00372.x
Subject(s) - circular dichroism , dna , biophysics , nanoparticle , fluorescence , ionic strength , chemistry , colloidal gold , conformational change , combinatorial chemistry , nanotechnology , crystallography , stereochemistry , materials science , biochemistry , organic chemistry , biology , aqueous solution , physics , quantum mechanics
A family of nanoparticles featuring surfaces of varying hydrophobicity was synthesized. The efficiency of DNA‐binding was determined, demonstrating in a fivefold modulation in binding a 37‐mer DNA strand. Nanoparticle‐binding causes a reversible conformational change in the DNA structure, as demonstrated by circular dichroism and fluorescence experiments. Furthermore, the affinity of the nanoparticle for the DNA can be regulated by external agents, though stability of the complex is observed at relatively high ionic strengths.