Open AccessExpression and localization of CKLFSF2 in human spermatogenesis
Author(s) -
Liu Gang,
Xin ZhongCheng,
Chen Liang,
Tian Long,
Yuan YiMing,
Song WeiDong,
Jiang XueJun,
Guo YingLu,
Giwercman Aleksander
Publication year - 2007
Publication title -
asian journal of andrology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.701
H-Index - 74
eISSN - 1745-7262
pISSN - 1008-682X
DOI - 10.1111/j.1745-7262.2007.00249.x
Subject(s) - endoplasmic reticulum , subcellular localization , biology , spermatogenesis , microbiology and biotechnology , golgi apparatus , in situ hybridization , spermiogenesis , meiosis , messenger rna , cytoplasm , gene , genetics , endocrinology
Aim: To investigate the expression and subcellular localization of chemokine‐like factor superfamily 2 (CKLFSF2) in human testis and its potential role in spermatogenesis. Methods: A specific polyclonal antibody against CKLFSF2 was raised. The expression and cellular localization of CKLFSF2 in the seminiferous tubules was checked by immunohistochemistry method. Also, in situ hybridization was applied to localize the mRNA distribution. The EGFP‐CKLFSF2 fusion protein was expressed in COS‐7 cells to localize its subcellular location in vitro. In addition, the abnormal expression of CKLFSF2 in testes of patients with male infertility was assayed by reverse transcription polymerase chain reaction (RT‐PCR) and immunohistochemistry methods. Results: Having a close correlation with spermatogenesis defects, CKLFSF2 was specifically expressed in meiotic and post‐meiotic germ cells, which were localized to the endoplasmic reticulum (ER) near the Golgi apparatus. Conclusion: CKLFSF2 could play important roles in the process of meiosis and spermiogenesis, and might be involved in the vesicular transport or membrane apposition events in the endoplasmic reticulum.