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Impact Assessment of Pumping Parameters on Groundwater Bacterial Communities
Author(s) -
Shani Noam,
Aravinthan Vasantha,
Rossi Pierre,
Laulan Guillaume,
Ducommun Pascale,
Holliger Christof
Publication year - 2012
Publication title -
groundwater monitoring and remediation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 47
eISSN - 1745-6592
pISSN - 1069-3629
DOI - 10.1111/j.1745-6592.2012.01420.x
Subject(s) - groundwater , aquifer , groundwater flow , environmental science , hydrology (agriculture) , lift (data mining) , water well , environmental engineering , volumetric flow rate , geotechnical engineering , geology , mechanics , physics , computer science , data mining
Groundwater microbial community samples are traditionally collected using pumping techniques optimized for groundwater chemistry assessment, although the impact of groundwater pumping parameters on apparent bacterial community structures ( BCSs ) is not really known. We therefore studied the impact of pumping lift, flow regime, and tubing material on BCS , which were analyzed by terminal‐restriction fragment length polymorphism (T‐ RFLP ). Ruzicka dissimilarity coefficients were calculated between T‐ RFLP profiles to assess disparities between BCS . Variations in pumping lift, flow regime, and tubing material did not affect the apparent BCS in experiments using a homogenous water system under laboratory conditions showing that the conditions within the tube had no detectable effect on BCS . However, pumping groundwater from aquifer monitoring wells at different flow rates in the field revealed a significant impact on the apparent BCS . Water samples collected from fine sediment were the most affected by the pumping flow rate.