Premium
GENETIC VARIABILITY OF TOXIGENIC AND NONTOXIGENIC ISOLATES OF ASPERGILLUS FLAVUS AND ASPERGILLUS PARASITICUS BY RAPD MARKER
Author(s) -
KHODADADI F.,
PANJEHKEH N.,
AHMADINEJAD M.,
AMINAEE M.M.
Publication year - 2011
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.2011.00319.x
Subject(s) - rapd , aspergillus flavus , aspergillus parasiticus , aflatoxin , biology , aspergillus , veterinary medicine , microbiology and biotechnology , genetic diversity , food science , population , medicine , environmental health
In this study, we determined the genetic relations between toxigenic and nontoxigenic Aspergillus flavus and Aspergillus parasiticus by random amplified polymorphic DNA (RAPD) markers. Eighteen samples from all 18 pistachio storages of Kerman City (the capital city of Kerman Province) were collected. A total of 51 fungi related to the flavi group were isolated, of which 17 were A. flavus , while the other 34 isolates were A. parasiticus . The amount and type of aflatoxin in all 51 samples were determined by high‐performance liquid chromatography method. Randomly six isolates of A. flavus and 13 isolates of A. parasiticus were selected for further molecular study. The DNA of these isolates was extracted and RAPD was done by five random primers. Data analysis showed that two of 19 isolates could not produce aflatoxin, and RAPD could not distinguish the toxigenic from nontoxigenic isolates but could distinguish A. flavus from A. parasiticus . On the other hand, in the phylogeny tree, two isolates of nontoxigenic fungi were among the toxigenic isolate. PRACTICAL APPLICATIONS Nontoxigenic isolates of Aspergillus flavus and Aspergillus parasiticus can be used as biological agents to control toxigenic isolates of the fungi.