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DETECTION OF CRONOBACTER IN INFANT FORMULA AND PHYLOGENETIC ANALYSIS ON α‐GLUCOSIDASE GENES
Author(s) -
YE YINGWANG,
WU QINGPING,
ZHANG JUMEI,
LU JIANFENG,
LIN LIN
Publication year - 2011
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.2010.00283.x
Subject(s) - cronobacter , phylogenetic tree , biology , infant formula , polymerase chain reaction , cronobacter sakazakii , gene , computational biology , phylogenetics , genetics , microbiology and biotechnology , food science , enterobacter , escherichia coli
The infant formula has been identified as the main transmission vehicle of Cronobacter infections that have been implicated in severe diseases in neonates. Therefore, the accurate detection of Cronobacter is required for microbiological safety of infant formulas. In this study, the duplex‐polymerase chain reaction (PCR) targeting α‐glucosidase genes was developed and could detect as few as 10 3 cfu/mL in artificially contaminated infant formulas. Positive signals were generated from Cronobacter but not from other non‐ Cronobacter . Newly developed dot hybridization further confirmed that the PCR was specific to detect Cronobacter in infant formula. Positive fragment sequencing was carried out for phylogenetic analysis and a high heterogeneity between Cronobacter and related species was observed. The phylogenetic analysis is helpful for enriching knowledge of genetic diversity and potential taxonomic complexity. PRACTICAL APPLICATION This new PCR method has a potential application for detection of Cronobacter in infant formulas prior to entry into markets and will be helpful in routine monitoring and risk assessment in large‐scale screening of disease outbreaks. Phylogenetic analysis is helpful for enriching knowledge of genetic diversity, further supporting and confirming the taxonomic complexity within Cronobacter .