Premium
EFFICACY OF HIGH HYDROSTATIC PRESSURE AND MILD HEAT TO REDUCE GEOBACILLUS STEAROTHERMOPHILUS AS 1.1923 SPORES IN MODEL FOOD SYSTEMS
Author(s) -
GAO YULONG
Publication year - 2010
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.2009.00194.x
Subject(s) - geobacillus stearothermophilus , hydrostatic pressure , spore , food science , endospore , pascalization , sterilization (economics) , hydrostatic equilibrium , response surface methodology , high pressure , matrix (chemical analysis) , chemistry , biology , thermodynamics , microbiology and biotechnology , chromatography , biochemistry , enzyme , thermophile , physics , quantum mechanics , monetary economics , economics , foreign exchange market , foreign exchange
The objective of this investigation was to evaluate the efficacy of high hydrostatic pressure and mild heat against spores of Geobacillus stearothermophilus AS 1.1923 in model food systems. The pressure‐processing conditions were fixed at 625.0 MPa and 86C for 14 min, which have been determined as the optimum processing conditions considering six‐log‐cycle reductions of G. stearothermophilus spores. Based on the results, response surface methodology was performed in the present investigation, the effects from food ingredients, such as soybean protein, soybean oil and sucrose, as well as pH of the food matrix on the inactivation of G. stearothermophilus spores by high pressure and mild heat was explored, and a quadratic predictive model for the effects of food ingredients and pH on the reduction levels of G. stearothermophilus spores by high‐pressure processing was built. The predictive model is significant because the level of significance was P < 0.0001 and the calculated F value is much greater than the tabulated F value. Moreover, the adequacy of the model equation for predicting the reduction of G. stearothermophilus spores was verified effectively.PRACTICAL APPLICATIONS This paper clearly demonstrated the contributions to pressure resistance from food ingredients such as soybean protein, soybean oil, sucrose and pH of the food matrix to the inactivation of Geobacillus stearothermophilus spores by high hydrostatic pressure and mild heat. The predictive model for predicting the reduction of G. stearothermophilus spores in model food systems was built which can be beneficial to targeted process development toward high‐pressure sterilization of foods.