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VIRULENCE LEVELS OF BIOFILM‐GROWN LISTERIA MONOCYTOGENES LO28 ARE LOWER THAN THOSE OF PLANKTONIC CELLS IN AN ORAL INOCULATION TEST ON MICE
Author(s) -
ZUNDEL ETIENNE,
GALLOIS YVES,
CONSTANTY EMMANUEL,
ROCHE SYLVIE M.,
MOMPART FLORENCE,
MEYLHEUC THIERRY,
PARDON PIERRE
Publication year - 2007
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.2007.00058.x
Subject(s) - virulence , listeria monocytogenes , biofilm , microbiology and biotechnology , inoculation , biology , bacteria , strain (injury) , listeria , immunology , biochemistry , genetics , anatomy , gene
The aim of this study was to produce Listeria monocytogenes biofilms suitable for virulence assays and to determine whether the released bacteria had the same virulence potential as their planktonic counterparts. Biofilms of Listeria monocytogenes LO28 strain, with or without Sphingomonas paucimobilis CCL10 strain, containing up to 7 log 10 cfu/cm 2 were produced in polypropylene syringes. The virulence of strain LO28 was analyzed in mice after intravenous, subcutaneous and oral inoculation. Its virulence level in binary cultures was not significantly different from that of monocultures. L. monocytogenes LO28 virulence in biofilms was lower than that of their planktonic counterparts after oral inoculation. Our results suggest that biofilms pose no greater health risk to the consumer than planktonic bacteria.