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EVIDENCE OF CROSS‐CONTAMINATION OF AEROMONAS HYDROPHILA BY FINGERPRINTING: SIGNIFICANCE FOR FOOD SAFETY
Author(s) -
GUNSALAM JURIN WOLMON,
RADU SON,
BENJAMIN PATRICK GUDA,
SELAMAT JINAP,
ROBIN TUNUNG
Publication year - 2006
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.2006.00050.x
Subject(s) - aeromonas hydrophila , rapd , biology , plasmid , microbiology and biotechnology , primer (cosmetics) , dna profiling , polymerase chain reaction , aeromonas , antibiotics , contamination , dna , genetics , bacteria , gene , genetic diversity , chemistry , ecology , population , demography , organic chemistry , sociology
The prevalence of Aeromonas hydrophila in a local wet market was studied and fingerprinting was used to prove the cross‐contamination. This study describes the use of antibiotic‐resistant plasmid profiles and random amplified polymorphic DNA (RAPD) assay to characterize strains of A. hydrophila. Almost all strains had multiple antibiotic resistances and carried small plasmid(s) of 1.4–6.0 MDa that enabled the A. hydrophila to be grouped into eight plasmid patterns. The RAPD‐polymerase chain reaction profiles observed after electrophoretic separation of the amplified products using primer GEN15007, which enable the A. hydrophila strains to be grouped into three clusters, demonstrate that the A. hydrophila strains were genetically heterogeneous. These results suggest that RAPD assay could be a valuable tool for epidemiological studies. In addition, antibiotic‐resistant and plasmid profiling can also be used as adjunct tools in this study.