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IN VITRO COMPARISON OF ANAEROBIC AND AEROBIC GROWTH RESPONSE OF SALMONELLA TYPHIMURIUM TO ZINC ADDITION
Author(s) -
PARK S.Y.,
WOODWARD C.L.,
BIRKHOLD S.G.,
KUBENA L.F.,
NISBET D.J.,
RICKE S.C.
Publication year - 2002
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.2002.tb00343.x
Subject(s) - anaerobic exercise , zinc , chemistry , food science , sulfate , salmonella , bacteria , biology , physiology , genetics , organic chemistry
Zinc supplemented diets have been used to provide zinc as a nutrient and higher concentrations have been used to induce molt in laying hens. It is not known if the zinc in these diets would inhibit Salmonella spp growth. This study examines the effects of zinc compounds on the growth of S. typhimurium poultry isolate under aerobic and anaerobic conditions. The aerobic growth response of S. typhimurium was determined either in tryptic soy broth (TSB) or minimal (M9) broth containing five different concentrations (0.67, 2.01, 3.35, 4.69, and 6.03% [wt/vol]) of either Zn acetate [Zn(C 2 H 2 O 2 ) 2 2H 2 O] or Zn sulfate [ZnSO 4 7H 2 O] while anaerobic growth response was determined in M9 broth with or without reductants (L‐cysteine hydrochloride [C 3 H 7 NO 2 SHCl], and sodium sulfide [Na 2 S 9H 2 O]). Aerobic growth rates inhibited (P < 0.05) by Zn acetate than by Zn sulfate in TSB medium. The Zn source and concentration decreased (P < 0.05) aerobic growth response of S. typhimurium poultry isolate in M9 medium. The growth rates of S. typhimurium under anaerobic growth conditions were less responsive to Zn salts but were generally lower (P < 0.05) in the presence of reductant than in the absence of reductants at each concentration of Zn compound. The results in this study provide evidence that Zn may inhibit S. typhimurium under in vitro aerobic or anaerobic atmospheric conditions and S. typhimurium grows less optimally under anaerobic growth conditions.