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DETECTION OF ANTIMICROBIAL DRUG RESIDUES IN KENYAN MILK
Author(s) -
SHITANDI ANAKALO,
STERNESJÖ ÅSE
Publication year - 2001
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.2001.tb00319.x
Subject(s) - penicillin , residue (chemistry) , antibiotics , antimicrobial , raw milk , food science , chromatography , agar diffusion test , microbiology and biotechnology , chemistry , biology , bacteria , antibacterial activity , biochemistry , genetics
The improved Dutch tube diffusion test was used to study the occurrence of inhibitory substances in raw bulk milk samples within the Nakuru District in Kenya. Initially the detection limits of the method were verified using milk standards spiked with selected antibiotics. Addition of penicillinase to inhibitor‐positive samples was used for preliminary identification of penicillin G‐type antibiotics and residue levels were estimated against a standard curve constructed by means of a B. stearothermophilus disc assay. The two‐tube test was used to screen 1109 field samples of which 229 (21%) were suspect positive. The identification procedure confirmed 165 samples (14.9%) to contain penicillin G‐type residues of which 118 contained levels exceeding the established EU MRL for penicillin G (4 μg/kg). This study indicates that antibiotic residues are prevalent in milk within the Nakuru district of Kenya. It suggests that the improved tube diffusion test in combination with a multiplate system could be useful for qualitative and quantitative identification of antimicrobial drug residues in milk.