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LACK OF CORRELATION BETWEEN PLASMID‐ASSOCIATED PHENOTYPES OF YERSINIA ENTEROCOLITICA AND PATHOGENICITY IN THE MOUSE 1
Author(s) -
BHADURI SAUMYA
Publication year - 1996
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.1996.tb00161.x
Subject(s) - virulence , plasmid , yersinia enterocolitica , biology , microbiology and biotechnology , phenotype , serotype , pathogenicity , pathogenicity island , yersinia , genetics , bacteria , dna , gene
Various phenotypic characteristics have been correlated with the pathogenicity of plasmid‐bearing virulent strains of Yersinia enterocolitica. Two transposon Tn 801‐insertion derivatives (JD193 and JD217) of the virulence plasmid from serotype 0:3 were used to determine the correlation between pathogenicity and plasmid‐associated properties of this organism. Both Tn 801‐inserted derivatives expressed five plasmid‐associated phenotypic characteristics at 37C: (1) colony morphology, (2) calcium‐dependent growth or low‐calcium response, (3) crystal violet binding, (4) autoagglutination, and (5) hydrophobicity. However, for mouse pathogenicity only JD193 was positive whereas, JD217 was avirulent for mice. Thus, it is possible to have a lack of correlation between plasmid‐mediated traits and the actual pathogenicity of the organism in the mouse; however, these plasmid‐mediated phenotypic characteristics provide simple and efficient techniques to evaluate the virulence potential of wild‐type strains isolated from food poisoning outbreaks and clinical cases .