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ISOLATION OF BDELLOVIBRIOS THAT PREY ON ESCHERICHIA COLI O157:H7 AND SALMONELLA SPECIES AND APPLICATION FOR REMOVAL OF PREY FROM STAINLESS STEEL SURFACES 1
Author(s) -
FRATAMICO PINA M.,
COOKE PETER H.
Publication year - 1996
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.1996.tb00157.x
Subject(s) - isolation (microbiology) , escherichia coli , salmonella , predation , microbiology and biotechnology , biology , chemistry , bacteria , ecology , biochemistry , paleontology , gene
Predatory bacteria of the genus Bdellovibrio that prey upon Escherichia coli O157:H7 and Salmonella species were isolated from soil and sewage samples. Bdellovibrio isolates 45k (from soil) and 88e (from sewage) attacked and lysed the E. coli and Salmonella strains tested, with log 10 reductions ranging from 2.5 to 7.9 CFU/mL after 7h incubation, using a two‐membered culture system. The predators showed activity against the bacteria within the temperature range of 19 to 37C. Bdellovibrio isolate 45k reduced a population of E. coli dried on stainless steel surfaces by 3.6 log 10 CFU/cm 2 following 24 h contact time and at a 10:1 predator to prey ratio and was also effective in reducing the level of biofilm cells. Bdellovibrios can potentially be utilized for removal of bacteria from surfaces of food processing equipment and possibly for controlling the growth of pathogenic and spoilage bacteria in foods.