Premium
OPTIMIZATION OF LISTERIOLYSIN O PRODUCTION BY LISTERIA MONOCYTOGENES 1
Author(s) -
KIM KEETAE,
MURANO ELSA A.,
OLSON DENNIS G.
Publication year - 1994
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.1994.tb00600.x
Subject(s) - listeriolysin o , listeria monocytogenes , hemolysis , serotype , incubation , microbiology and biotechnology , lysis , biology , incubation period , bacteria , chemistry , listeria , biochemistry , immunology , genetics
Listeria monocytogenes serotype 1 and Scott A strains were examined to determine the effect of culture conditions onproduction of listeriolysin O (LLO). The hemolytic activity was assayed by hemolysis of sheep red blood cells. The optimum pH range for LLO production in serotype 1 and Scott A were pH 6.2–6.0 and pH 5.8–6.0, respectively. Production of LLO by serotype 1 was 20–fold higher than by Scott A. Addition of more than 1.0% glucose significantly reduced production of LLO by the cells, while favoring growth. By adding 0.5% glucose after stationary phase and adjusting pH to 5.5–7.0, maximum LLO was produced after 2 h incubation at 37C by both strains.