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DETECTION OF FIMBRIAE (CFA/I) ON ENTEROTOXIGENIC ESCHERICHIA COLI USING ANTIBODIES
Author(s) -
SINGLETON EMMA R.,
HAMDY MOSTAFA K.,
McCAY STEVEN G.,
ZAPATKA FRANCIS A.
Publication year - 1990
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.1990.tb00053.x
Subject(s) - fimbria , enterotoxigenic escherichia coli , polyclonal antibodies , escherichia coli , antiserum , microbiology and biotechnology , biology , antigen , isoelectric point , gene , biochemistry , enterotoxin , genetics , enzyme
Fimbriae (CFA/I) were isolated after detachment from an enterotoxigenic Escherichia coli ( ETEC) strain H10407, purified by successive isoelectric point precipitation at pH 3.5, and injected into rabbits. The antisera containing the polyclonal antibodies against the fimbrial proteins (antigens) were then used to detect the presence of CFA/I on other E. coli cultures using an indirect ELISA system. The ELISA procedures for the CFA/I assay was as sensitive as the DNA hybridization for detecting the ETEC strains. Two bands (major and minor) were present when the isolated fimbrial proteins were subjected to SDS‐PAGE. The major band had a molecular mass of 40.3 and the minor 36.4 kDa. Electron microscopy of the fimbriae appeared as thin thread rods with variable length (5–15 μm ).