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EXTINCTION OF LISTERIA MONOCYTOGENES IN SINGLE‐STRENGTH ORANGE JUICE: COMPARISON OF METHODS FOR DETECTION IN MIXED POPULATIONS 1
Author(s) -
PARISH M. E.,
HIGGINS D. P.
Publication year - 1989
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.1989.tb00526.x
Subject(s) - listeria monocytogenes , orange juice , listeria , food science , agar , chemistry , microbiology and biotechnology , biology , bacteria , genetics
The FDA method of selective enrichment followed by selective plating on modified McBride agar was capable of detecting the presence of L. monocytogenes inoculated into a typical, commerical, reconstituted, single‐strength orange juice at the 10 0 cfu/mL level. The KOH shock treatment, formerly recommended in the FDA protocol, negatively affected recovery of Listeria at 10 0 and 10 1 levels in juice which also had a low background microflora (10 2 cfu/mL total count); however, KOH treatment was required for Listeria detection in juice which had high background microflora (10 8 cfu/mL). Two other protocols for detection of Listeria which utilized cold enrichment or different selective enrichment media were less effective than the FDA procedure due to heavy growth of background microflora. No Listeria was detected in 100 commerical orange juice samples from dairy and nondairy processors in geographically distinct areas of North America using the FDA method.