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DETECTION OF BACILLUS CEREUS DIARRHEAGENIC TOXIN USING A RAT LIGATED INTESTINAL LOOP ASSAY
Author(s) -
TING WEITSYI,
BANWART GEORGE J.
Publication year - 1985
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.1985.tb00494.x
Subject(s) - bacillus cereus , toxin , microbiology and biotechnology , cereus , chemistry , biology , chromatography , bacteria , genetics
A study was performed to determine if the rat ligated intestinal loop assay could detect Bacillus cereus diarrheagenic toxin. The results obtained indicated that this assay system could be used for such a purpose but it was not as sensitive as the young rabbit ligated ileal loop assay. When rats were used, concentrating the cell free culture filtrate of B. cereus 30‐ to 40‐fold was necessary to detect diarrheagenic toxin activity with the intestinal loop assay.