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NITRITE REDUCTASE IN SALMONELLA TYPHIMURIUM
Author(s) -
PAGE GREGORY V.,
SOLBERG MYRON,
CARMAN GEORGE M.
Publication year - 1985
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.1985.tb00489.x
Subject(s) - nitrite , nitrite reductase , biochemistry , cofactor , chemistry , fermentation , reductase , anaerobic exercise , enzyme , salmonella , microbiology and biotechnology , biology , food science , bacteria , nitrate reductase , nitrate , organic chemistry , genetics , physiology
A nitrite reductase was identified and purified 11 ‐fold from the cytoplasmic fraction of Salmonella typhimurium grown anaerobically with nitrite as the sole nitrogen source. The enzyme required NADH as a cofactor and showed maximum in vitro reductase activity at pH 8.0. S. typhimurium, grown anaerobically in glucose‐limited minimal medium containing peptone and nitrite, showed shorter generation times and increased cell yields in comparison to nitrite‐free cultures. The presence of nitrite had no effect on aerobic cultures. The nitrite reductase functions in a dissimilatory manner and appears to be primarily involved in physiological energy generation during anaerobic growth. The nitrite reductase may function to remove excess reducing power in the form of NADH from the cell thus increasing ATP production during the anaerobic fermentation of glucose. The formation and subsequent assimilation of ammonia from nitrite is of secondary importance to the organism.