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IMMUNODIFFUSION METHOD FOR DETECTION OF CLOSTRIDIUM BOTULINUM TYPES, A, B, E, AND F
Author(s) -
FERREIRA J. L.,
HAMDY M. K.,
ZAPATKA F. A.,
HEBERT W. O.
Publication year - 1983
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.1983.tb00459.x
Subject(s) - clostridium sporogenes , clostridium botulinum , precipitin , agar gel , microbiology and biotechnology , immunodiffusion , agar , clostridiaceae , ouchterlony double immunodiffusion , titer , chemistry , biology , clostridium , antigen , antibody , bacteria , immunology , toxin , antiserum , genetics
Factors affecting the immuno‐gel diffusion method for detecting toxigenic (tox + ) C. botulinum type A and nontoxigenic (tax ‐ ) C. sporogenes were studied. This procedure was extended to detect types B, E, and F using homologous and poly A‐F antitoxins for proper tox ‐ types. Increasing glucose levels from 0 to 3% in the growth medium caused larger and more intense precipitin zones around colonies of C. botulinum type A. Precipitin zones were detected in TPGYA that contained no glucose, but better zones occurred at 4% and thereafter up to 7% glucose at pH 7.6. The most favorable titers of C. botulinum antitoxins incorporated either in gel‐diffusion agar (GDA) or in growth medium varied with the C. botulinum type. The method differentiates between C. botulinum types A, B, E, F and C. sporogenes.