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PURIFICATION AND PROPERTIES OF DESMUTAGENIC FACTOR FROM BROCCOLI ( BRASSICA OLERANCEA VAR. ITALICA PLENCK ) FOR MUTAGENIC PRINCIPLE OF TRYPTOPHAN PYROLYSATE
Author(s) -
MORITA KAZUYOSHI,
YAMADA HIROKAZU,
IWAMOTO SEIICHI,
SOTOMURA MIKIO,
SUZUKI AKIRA
Publication year - 1982
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.1982.tb00438.x
Subject(s) - indole test , tryptophan , chemistry , chromatography , brassica , potassium , centrifugation , absorption (acoustics) , elution , cellulose , amino acid , biochemistry , organic chemistry , biology , botany , acoustics , physics
A desmutagenic factor which inhibited the mutagenicity of the mutagens, Trp‐P‐1 (3‐amino‐1,4‐dimethyl‐5H‐pyrido[4,3‐b]indole), Trp‐P‐2 (3‐amino‐1‐methyl)‐5H‐pyrido[4,3‐b]indole), ethidiumbromide and 2‐aminoanthracene, was purified from broccoli (Brassica olerancea var. italica plenck). The factor was not sedimented by ultra‐centrifugation at 200,000 xg for 2 h. It adsorbed to a DEAE‐cellulose column and was eluted with low concentration of potassium chloride. The purified factor exhibited a heme‐like protein absorption spectrum with a Soret band at 403 nm and α and β bands at 640 and 504 nm, respectively. The molecualr weight was estimated to be approximately 53,000 by SDS‐gel electrophoresis.