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SUITABILITY OF CYNOMOLOGUS MONKEYS ( MACACA FASCICULARIS ) FOR STAPHYLOCOCCAL ENTEROTOXIN BIOASSAY
Author(s) -
ADESIYUN A. A.,
TATINI S. R.
Publication year - 1981
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.1981.tb00421.x
Subject(s) - bioassay , enterotoxin , antiserum , biology , microbiology and biotechnology , chemistry , antibody , immunology , biochemistry , ecology , escherichia coli , gene
Cynomologus monkeys (Macaca fascicularis) were tested for their sensitivity and specificity to staphylococcal enterotoxin A (SEA). Thirty‐two of 38 monkeys vomited within 5 h in response to intragastric feeding of 4.8–18 μg of crude SEA. Twenty‐four of these 32 responding monkeys were subjected to specificity study by feeding crude SEA which was neutralized with specific Antiserum A. Twenty‐two (92%) of the 24 demonstrated specificity by not vomiting when fed neutralized crude SEA. The remaining two (8%) monkeys showed specificity only with purified SEA neutralized with the Antiserum. The emetic dose ‐50 for crude SEA was 6.5 μg per monkey. These suggest that cynomologus monkeys are suitable for SE bioassay and for identification of new enterotoxins.