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DETERMINATION OF THE EFFECTIVENESS OF NOVOBIOCIN ADDED TO TWO AGAR PLATING MEDIA FOR THE ISOLATION OF SALMONELLA FROM FRESH MEAT PRODUCTS
Author(s) -
KOMATSU KEN K.,
RESTAINO L.
Publication year - 1981
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.1981.tb00420.x
Subject(s) - agar , citrobacter freundii , enterobacter , proteus mirabilis , novobiocin , sulfadiazine , microbiology and biotechnology , biology , citrobacter , salmonella , food science , enterobacteriaceae , isolation (microbiology) , escherichia coli , bacteria , pseudomonas aeruginosa , antibiotics , biochemistry , genetics , gene
Five plating media, Hektoen enteric (HE) and xylose lysine deoxycholate (XLD) agars with and without 80 and 5 μg/ml of novobiocin (N), respectively, and brilliant greeen sulfadiazine (BGS) agar with 80 μg/ml of the antimicrobial agent, were analyzed for the recovery of salmonellae from various fresh beef, pork, and poultry meat products. Of the total Samonella positive samples, 50.0% and 82.5% were found on XLD and XLD‐N agars, respectively, 75.0% and 85.0% on HE and HE‐N agars, respectively and 65.0% on BGS agar. HE‐N and BGS media isolated three times more false positives than did XLD‐N agar, while XLD and HE agars gave the highest numbers of false positives. The major H 2 S producing false positive on XLD and HE agars was Proteus mirabilis. With the addition of N, P. mirabilis was eliminated, and the major H 2 S producing false positive was almost exclusively Citrobacter freundii. The false positives on BGS agar were predominately distributed among C. freundii, Enterobacter sp., and Klebsiella sp.