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PREPARATION AND CHARACTERIZATION OF AFLATOXIN B 2a ‐HEMIGLUTARATE AND ITS USE FOR THE PRODUCTION OF ANTIBODY AGAINST AFLATOXIN B 1
Author(s) -
LAU H. P.,
GAUR P. K.,
CHU F. S.
Publication year - 1980
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.1980.tb00404.x
Subject(s) - aflatoxin , bovine serum albumin , chemistry , aqueous solution , nuclear chemistry , hydrolysis , chromatography , organic chemistry , food science
Hemisuccinate (HS) and hemiglutarate (HG) of aflatoxin B 2a (afla B 2a ) were prepared by refluxing afla B 2a with the corresponding anhydride and 4‐N, N‐dimethylaminopyridine in tetrahydrofuran. Two epimers of the respective HS or HG which show different chromatographic behavior and physiochemical properties were isolated and characterized. Afla B 2a ‐HS hydrolyzes very rapidly in aqueous solution and was not used for further study. Afla B 2a ‐HG hydrolyzes at a much slower rate and was selected for the coupling to protein. Using the mixed anhydride method, as much as 12 moles of afla B 2a ‐HG were conjugated to each mole of bovine serum albumin (BSA). The antibody obtained from rabbits immunized with afla B 2a ‐HG BSA is most specific to afla B 1 and shows little cross reaction with afla G 1 and aflatoxicol. The lower limit for detection of afla B 1 by radioimmunoassay using this antibody is in the range of 30–50 pg per assay.