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INTERFERENCE BY ASCORBIC ACID IN THE DETERMINATION OF NITRITE BY THE SEPARATE COLOR REAGENT ADDITION TECHNIQUE
Author(s) -
SEN N. P.,
McPHERSON M.
Publication year - 1978
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.1978.tb00279.x
Subject(s) - ascorbic acid , nitrite , reagent , chemistry , sulfanilamide , diazo , color reaction , chromatography , colorimetry , incubation , organic chemistry , food science , biochemistry , nitrate
A study was carried out to determine if the presence of ascorbic acid interferes with the colorimetric determination of nitrite by the technique of separate addition of color reagents. It was shown that ascorbate can destroy some of the nitrite during pre‐incubation with sulfanilamide, one of the color reagents used to form the diazo compound. An alternative method of analysis is suggested in which ascorbic acid, in the concentration range normally found in foods and cured meat products, does not interfere.