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RELEASE OF ENTEROTOXIN A AND THERMONUCLEASE FROM GROWING AND NON‐GROWING CELLS OF STAPHYLOCOCCUS AUREUS
Author(s) -
NISKANEN AIMO
Publication year - 1977
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.1977.tb00264.x
Subject(s) - enterotoxin , staphylococcus aureus , intracellular , microbiology and biotechnology , chemistry , toxin , biology , biochemistry , bacteria , escherichia coli , gene , genetics
Enterotoxin A and thermonuclease were produced by Staphylococcus aureus cell populations in all growth phases. Synthesis of entero‐toxin was more rapid than that of thermonuclease early in the exponential phase, whereas later in the exponential phase the thermonuclease synthesis was more rapid than that of enterotoxin. The optimum temperature for enterotoxin production was 42°C, while that for thermonuclease production was in the range 27–33°C. Small amounts of enterotoxin and thermonuclease were released at temperatures up to 45°C. During heat treatment of staphylococcal cells, enterotoxin production was not observed at temperatures in excess of those permitting growth, although small amounts of thermonuclease were produced. Intracellular enterotoxin was not detected after disintegration of cells under pressure at –30°C. Enterotoxin at detectable concentration, was not bound to the cell membranes. All the results obtained indicate that the synthesis of enterotoxin by S. aureus is intimately associated with cell growth.