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PRODUCTION OF STEROID GLYCOALKALOIDS BY PHYTOPHTHORA INFESTANS IN COMPLEX AND CHEMICALLY DEFINED MEDIA
Author(s) -
MAAS MELANIE R.,
POST F. J.,
SALUNKHE D. K.
Publication year - 1977
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.1977.tb00263.x
Subject(s) - glycoalkaloid , phytophthora infestans , mycelium , maltose , distilled water , dry weight , food science , chemistry , incubation , botany , chemically defined medium , growth medium , horticulture , biology , biochemistry , chromatography , sucrose , in vitro , solanaceae , blight , bacteria , genetics , gene
A chemically defined synthetic medium was developed on which Phytophthora infestans (Mont.) de Bary strain 1.2.4 grew extensively. The medium consisted of: 90.0 g maltose; 20.0 g L‐proline; 20.0 g DL‐alanine; 1.0 g KNO 3 ; 0.50 g KH 2 PO 4 ; 0.25 g MgSO 4 7H 2 O; and 1.0 mg thiamin per liter of distilled water. The average level of growth on this medium was 0.5864 g dry weight mycelium per 50 ml medium. The glycoalkaloids, solanidine and solanine, were produced by P. infestans in this medium at an average concentration of 0.8518 mg total glycoalkaloids per 25 ml medium with a range of 0.0 to 5.9 mg per 25 ml. No glycoalkaloid production was detected when the level of growth of the fungus was below 0.35 g dry weight mycelium/50 ml medium. Glycoalkaloids were synthesized by 5 of 10 cultures grown on chick pea medium and by none of 10 cultures grown on rye seed medium.