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A SIMPLE SOLID‐PHASE RADIOIMMUNOASSAY FOR AFLATOXIN B 1
Author(s) -
SUN PIERA S.,
CHU F. S.
Publication year - 1977
Publication title -
journal of food safety
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.427
H-Index - 43
eISSN - 1745-4565
pISSN - 0149-6085
DOI - 10.1111/j.1745-4565.1977.tb00260.x
Subject(s) - chromatography , radioimmunoassay , chemistry , sepharose , size exclusion chromatography , biochemistry , enzyme
A solid‐phase radioimmunoassay (RIA) for aflatoxin B 1 (afla B 1 ) was developed. This method involved the incubation of afla B 1 , both labelled and unlabelled, with immunoglobulin (IgG)‐sepharose gel which was prepared by conjugation of the IgG highly specific to afla B 1 with CNBr‐activated sepharose gel, followed by a filtration step. The binding capacity was determined by counting the radioactivity in the filtrate. Studies with different afla B 1 analogues revealed that the IgG‐gel bound most effectively with B 1 . Binding of afla B 2 , G 1 , G 2 , and aflatoxicol to the IgG‐gel was less effective in comparison with the IgG before coupling. Between 0.5–5.0 ng per assay, the displacement of radioactivity from the gel was directly proportional to the amount of afla B 1 present. Using a simple extraction procedure without clean‐up step, the recovery yields for afla B 1 in the contaminated corn or wheat at levels of 5 ppb or above were above 60%.