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MEMBRANE FILTRATION FOR ISOLATION, FRACTIONATION AND PURIFICATION OF FOOD‐AND FEED‐GRADE PROTEINS FROM PASTURE HERBAGE
Author(s) -
OSTROWSKI HENRY T.
Publication year - 1978
Publication title -
journal of food processing and preservation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.511
H-Index - 48
eISSN - 1745-4549
pISSN - 0145-8892
DOI - 10.1111/j.1745-4549.1978.tb00571.x
Subject(s) - ultrafiltration (renal) , chemistry , dry matter , fractionation , chromatography , filtration (mathematics) , microfiltration , membrane , agronomy , biochemistry , biology , statistics , mathematics
In 3 trials a comparison of the membrane filtration (ultrafiltration — UF) efficiency of 3 different ultrafiltration systems (ABCOR, ROMICON AND DDS) has been made with an evaluation of the feasibility of the procedure for its application for protein recovery from juices extracted from pasture herbage as an alternative to heat and acid precipitation. The ABCOR system was the most convenient for a batch system of herbage juices within a wide range of dry matter content (4 to 11% DM). The Romicon hollow fiber system had a much higher ultrafiltration efficiency compared to the ABCOR and DDS UF units but was subject to spontaneous mechanical failure – blockage and breakage of the hollow fibers. Five protein fractions were observed in pasture herbage juices with the major 3 being separated routinely in practice. Protein fractions concentrated by the ultrafiltration after spray drying were still water soluble (dispersable). This compares with an insoluble product obtained as a result of heat precipitation. The ultrafiltration conditions (dry matter and protein content, temperature, recirculation rate, pressure, pH, size of plant) are critical or the efficiency of DM and protein concentration in the final product recovered from herbage juice in a plant scale operation so there is a need for their careful standardization and optimization.