Premium
OPTIMIZATION OF ANGIOTENSIN I‐CONVERTING ENZYME INHIBITORY PEPTIDE PRODUCTION BY RIBBONFISH ( TRICHIURUS HAUMELA ) BACKBONE HYDROLYSIS USING RESPONSE SURFACE METHODOLOGY
Author(s) -
WANG JINLING,
HE GUOQING,
CHEN QIHE,
FENG FENGQIN,
YU GUANGJIAN,
SHOEMAKER CHARLES F.
Publication year - 2011
Publication title -
journal of food process engineering
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 45
eISSN - 1745-4530
pISSN - 0145-8876
DOI - 10.1111/j.1745-4530.2009.00524.x
Subject(s) - response surface methodology , hydrolysis , protease , chemistry , central composite design , substrate (aquarium) , enzyme , hydrolysate , enzymatic hydrolysis , chromatography , peptide , fractional factorial design , factorial experiment , biochemistry , biology , mathematics , ecology , statistics
The effect of an acid protease hydrolysis on protein from ribbonfish backbone to produce peptides with angiotensin I‐converting enzyme inhibitory activity (ACEIP) was studied and optimized using response surface methodology (RSM). To optimize the hydrolysis reaction, a three‐step design was used which included fractional factorial design, steepest ascent design and central composite design (CCD). The results showed that the optimal conditions of ribbonfish backbone protein hydrolysis to produce ACEIP were: pH of 2.67, temperature of 45C, enzyme to substrate ratio of 1/150 (g/g protein), substrate to water ratio of 1:4.38 (g/mL) and hydrolysis time period of 7.0 h. With these conditions, the hydrolyzate concentration (mg/mL) that inhibited 50% angiotensin I‐converting enzyme activity (ACEIC 50 ) was 1.054 ± 0.016 mg/mL, which compared well with the predicted value (1.050 mg/mL) by RSM model.PRACTICAL APPLICATIONS Ribbonfish is one of the principle catches from the China Sea. It is processed into various fish products in many factories in the China coastal areas. However, significant amounts of the ribbonfish backbone (12% protein, wet weight) are by‐products or waste from the processing, which are not fully utilized. It is often used as animal feedstuff. This study presents a use of the ribbonfish backbone to produce bioactive peptides with angiotensin I‐converting enzyme inhibitory activity, through optimizing an acid protease hydrolysis on protein from ribbonfish backbone using response surface methodology.